E01B0003 Human β Defensin 3 ELISA kit
The Human β Defensin 3 ELISA kit can be used to identify samples from the human species. β Defensin 3 can also be called DEFb3, Beta-defensin 3, BNBD-3, BNDB-3, DEFB3, BNDB3.
Specifications of Human β Defensin 3 ELISA kit
Product Information | |
Cat. No. | E01B0003 |
Product Name | Human β Defensin 3 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DEFb3 ELISA kit uses an anti-DEFb3 antibody and an DEFb3-HRP conjugate in a competitive enzyme immunoassay method. DEFb3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DEFb3 antibody binding site between DEFb3 from samples and DEFb3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DEFb3. Since the number of sites is limited, as more sites are occupied by DEFb3 from the sample, fewer sites are left to bind DEFb3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFb3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human β Defensin 3 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DEFb3 and analogues was observed. | |
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Summary of the Assay Procedure for Human β Defensin 3 ELISA kit
Citations of Human β Defensin 3 ELISA kit
E01B0003 has been referenced in the below publications：
Influences of inhaling glucocorticoid on sIgA and HBD-2,3 in pharyngeal mucosa of and T cell subsets in blood of patients with chronic persistent asthma.
