E01I0770 Human 8-Isoprostane ELISA kit
The Human 8-Isoprostane ELISA kit can be used to identify samples from the human species. 8-Isoprostane can also be called 8-iso-PGF2α.
Specifications of Human 8-Isoprostane ELISA kit
Product Information | |
Cat. No. | E01I0770 |
Product Name | Human 8-Isoprostane ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
8-iso-PGF 2α ELISA kit uses an anti-8-iso-PGF 2α antibody and an 8-iso-PGF 2α-HRP conjugate in a competitive enzyme immunoassay method. 8-iso-PGF 2α-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-8-iso-PGF 2α antibody binding site between 8-iso-PGF 2α from samples and 8-iso-PGF 2α-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 8-iso-PGF 2α. Since the number of sites is limited, as more sites are occupied by 8-iso-PGF 2α from the sample, fewer sites are left to bind 8-iso-PGF 2α-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 8-iso-PGF 2α concentration in each sample is interpolated from this standard curve. |
Quality Control on Human 8-Isoprostane ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 8-iso-PGF 2α and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human 8-Isoprostane ELISA kit
Citations of Human 8-Isoprostane ELISA kit
E01I0770 has been referenced in the below publications：
Oxidative stress and antioxidant status in women with gestational diabetes mellitus diagnosed by IADPSG criteria.
