Human CD147/EMMPRIN ELISA kit (E01C0234) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human CD147/EMMPRIN ELISA kit (E01C0234)

E01C0234 Human CD147/EMMPRIN ELISA kit ELISA kit

The Human CD147/EMMPRIN ELISA kit can be used to identify samples from the human species. CD147/EMMPRIN can also be called CD147, TCSF, Emmprin, 5A11 antigen, 5F7, Basigin, Blood brain barrier HT7 antigen, BSG, CD 147, CD147 antigen, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M6, M6 leukocyte activation antigen, neurothelin,TCSF, Tumor cell derived collagenase stimulatory factor.

Products

Specifications of Human CD147/EMMPRIN ELISA kit

Product Information
Cat. No.	E01C0234
Product Name	Human CD147/EMMPRIN ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	100-2500 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	100 pg/mL	1 vial
STANDARD C (0.5mL)	250 pg/mL	1 vial
STANDARD D (0.5mL)	500 pg/mL	1 vial
STANDARD E (0.5mL)	1000 pg/mL	1 vial
STANDARD F (0.5mL)	2500 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CD147 ELISA kit uses an anti-CD147 antibody and an CD147-HRP conjugate in a competitive enzyme immunoassay method. CD147-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CD147 antibody binding site between CD147 from samples and CD147-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CD147. Since the number of sites is limited, as more sites are occupied by CD147 from the sample, fewer sites are left to bind CD147-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD147 concentration in each sample is interpolated from this standard curve.

Quality Control on Human CD147/EMMPRIN ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CD147 and analogues was observed.