E01B0030 Human Anti immunobulin Binding Protein antibody ELISA kit
The Human Anti immunobulin Binding Protein antibody ELISA kit can be used to identify samples from the human species. Anti immunobulin Binding Protein antibody can also be called Immunoglobulin-binding protein antibody,Binding immunoglobulin protein antibody.
Product Information | |
Cat. No. | E01B0030 |
Product Name | Human Anti immunobulin Binding Protein antibody ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 10 ug/mL | 1 vial |
STANDARD D (0.5mL) | 25 ug/mL | 1 vial |
STANDARD E (0.5mL) | 50 ug/mL | 1 vial |
STANDARD F (0.5mL) | 100 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Anti-BIP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Anti-BIP antibody and an Anti-BIP-HRP conjugate. The assay sample and buffer are incubated together with Anti-BIP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-BIP concentration since Anti-BIP from samples and Anti-BIP-HRP conjugate compete for the anti-Anti-BIP antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti-BIP from the sample, fewer sites are left to bind Anti-BIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-BIP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TIG and analogues was observed. | |
E01B0030 has been referenced in the below publications：
Diagnostic value of autoantibodies combined detection for rheumatoid arthritis.
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