Human C-lara cell secretory protein 10 ELISA kit (E01C2721)

BlueGene Biotech Human C-lara cell secretory protein 10 ELISA kit (E01C2721)

E01C2721 Human C-lara cell secretory protein 10 ELISA kit

The Human C-lara cell secretory protein 10 ELISA kit can be used to identify samples from the Human species. C-lara cell secretory protein 10 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1, uteroglobin, uteroglobin-like antigen, UGL, club-cell secretory protein, Clara-cell 16 kD protein, club-cell-specific 10 kD protein, urine protein 1, polychlorinated biphenyl-binding protein, PCB-BP, club cell phospholipid-binding protein, UG, Uteroglobin, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein, Secretoglobin Family 1A Member 1, Clara-Cell Specific 10-kD Protein, blastokinin.

Products

Specifications of Human C-lara cell secretory protein 10 ELISA kit

Product Information
Cat. No.	E01C2721
Product Name	Human C-lara cell secretory protein 10 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CC-10 ELISA kit uses an anti-CC-10 antibody and an CC-10-HRP conjugate in a competitive enzyme immunoassay method. CC-10-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CC-10 antibody binding site between CC-10 from samples and CC-10-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CC-10. Since the number of sites is limited, as more sites are occupied by CC-10 from the sample, fewer sites are left to bind CC-10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CC-10 concentration in each sample is interpolated from this standard curve.

Quality Control on Human C-lara cell secretory protein 10 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CC-10 and analogues was observed.