E01C0613 Human Cyclophilin A ELISA kit
The Human Cyclophilin A ELISA kit can be used to identify samples from the human species. Cyclophilin A can also be called Cyclophilin A, Cyclosporin A binding protein, CYPH, Peptidyl prolyl cis trans isomerase A, Peptidyl-prolyl cis-trans isomerase A, Peptidylprolyl isomerase A, PPIA, PPIA protein, PPIase A, Rotamase A, T cell cyclophilin.
Product Information | |
Cat. No. | E01C0613 |
Product Name | Human Cyclophilin A ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 10 ug/mL | 1 vial |
STANDARD D (0.5mL) | 25 ug/mL | 1 vial |
STANDARD E (0.5mL) | 50 ug/mL | 1 vial |
STANDARD F (0.5mL) | 100 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CYPA ELISA kit uses an anti-CYPA antibody and an CYPA-HRP conjugate in a competitive enzyme immunoassay method. CYPA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CYPA antibody binding site between CYPA from samples and CYPA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CYPA. Since the number of sites is limited, as more sites are occupied by CYPA from the sample, fewer sites are left to bind CYPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYPA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CYPA and analogues was observed. | |
E01C0613 has been referenced in the below publications：
Expressions of EMMPRIN and its ligand CyPA in gingival crevicular fluid of chronic periodontitis patients.
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