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  • bovine serum albumin elisa 1

BlueGene Biotech Human Cyclophilin A ELISA kit (E01C0613)

E01C0613 Human Cyclophilin A ELISA kit

The Human Cyclophilin A ELISA kit can be used to identify samples from the human species. Cyclophilin A can also be called Cyclophilin A, Cyclosporin A binding protein, CYPH, Peptidyl prolyl cis trans isomerase A, Peptidyl-prolyl cis-trans isomerase A, Peptidylprolyl isomerase A, PPIA, PPIA protein, PPIase A, Rotamase A, T cell cyclophilin.

Products

Specifications of Human Cyclophilin A ELISA kit

Product Information

Cat. No.

E01C0613

Product Name

Human Cyclophilin A ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ug/ml

Sensitivity

1.0 ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

5.0 ug/mL

1 vial

STANDARD C (0.5mL)

10 ug/mL

1 vial

STANDARD D (0.5mL)

25 ug/mL

1 vial

STANDARD E (0.5mL)

50 ug/mL

1 vial

STANDARD F (0.5mL)

100 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CYPA ELISA kit uses an anti-CYPA antibody and an CYPA-HRP conjugate in a competitive enzyme immunoassay method. CYPA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CYPA antibody binding site between CYPA from samples and CYPA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CYPA. Since the number of sites is limited, as more sites are occupied by CYPA from the sample, fewer sites are left to bind CYPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYPA concentration in each sample is interpolated from this standard curve.


Quality Control on Human Cyclophilin A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CYPA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Cyclophilin A ELISA kit

Summary of the Assay Procedure for Human Cyclophilin A ELISA kit

Citations of Human Cyclophilin A ELISA kit

E01C0613 has been referenced in the below publications:

Expressions of EMMPRIN and its ligand CyPA in gingival crevicular fluid of chronic periodontitis patients.


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