E01C0535 Human Calcineurin ELISA kit
The Human Calcineurin ELISA kit can be used to identify samples from the human species. Calcineurin can also be called CaN, Calcineurin, protein phosphatase 3, calcium-dependent serine-threonine phosphatase.
Specifications of Human Calcineurin ELISA kit
Product Information | |
Cat. No. | E01C0535 |
Product Name | Human Calcineurin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CaN ELISA kit uses an anti-CaN antibody and an CaN-HRP conjugate in a competitive enzyme immunoassay method. CaN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CaN antibody binding site between CaN from samples and CaN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CaN. Since the number of sites is limited, as more sites are occupied by CaN from the sample, fewer sites are left to bind CaN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CaN concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Calcineurin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CaN and analogues was observed. | |
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Summary of the Assay Procedure for Human Calcineurin ELISA kit
Citations of Human Calcineurin ELISA kit
E01C0535 has been referenced in the below publications：
Role of the NF-κB pathway in dibutyl phthalate mediated effects in U251 cells.
