E01C0004 Human Cluster of Differentiation 4 ELISA kit
The Human Cluster of Differentiation 4 ELISA kit can be used to identify samples from the bovine species. Cluster of Differentiation 4 can also be called CD4 (L3T4), CD4 antigen (p55), CD4 Antigen , CD4 molecule, CD4 Receptor, CD4+ Lymphocyte deficiency, included, CD4mut.
Product Information | |
Cat. No. | E01C0004 |
Product Name | Human Cluster of Differentiation 4 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CD4 ELISA kit uses an anti-CD4 antibody and an CD4-HRP conjugate in a competitive enzyme immunoassay method. CD4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CD4 antibody binding site between CD4 from samples and CD4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CD4. Since the number of sites is limited, as more sites are occupied by CD4 from the sample, fewer sites are left to bind CD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD4 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CD4 and analogues was observed. | |
E01C0004 has been referenced in the below publications：
Rosiglitazone negatively regulates c-Jun N-terminal kinase and toll-like receptor 4 proinflammatory signalling during initiation of experimental aortic aneurysms.
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