Human Complement Component 3 ELISA kit (E01C0360) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Complement Component 3 ELISA kit (E01C0360)

E01C0360 Human Complement Component 3 ELISA kit

The Human Complement Component 3 ELISA kit can be used to identify samples from the human species. Complement Component 3 can also be called C3, AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement component 3, Acylation-stimulating protein cleavage product, ASP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, Complement C3 alpha chain, Complement C3, Complement C3 beta chain, Complement C3 precursor, Complement C3b alpha chain, Complement C3c fragment, Complement component 3.

Products

Specifications of Human Complement Component 3 ELISA kit

Product Information
Cat. No.	E01C0360
Product Name	Human Complement Component 3 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 mg/L
Sensitivity	0.1 mg/L
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 mg/L	1 vial
STANDARD B (0.5mL)	1.0 mg/L	1 vial
STANDARD C (0.5mL)	2.5 mg/L	1 vial
STANDARD D (0.5mL)	5.0 mg/L	1 vial
STANDARD E (0.5mL)	10 mg/L	1 vial
STANDARD F (0.5mL)	25 mg/L	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

C3 ELISA kit uses an anti-C3 antibody and an C3-HRP conjugate in a competitive enzyme immunoassay method. C3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-C3 antibody binding site between C3 from samples and C3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of C3. Since the number of sites is limited, as more sites are occupied by C3 from the sample, fewer sites are left to bind C3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Complement Component 3 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between C3 and analogues was observed.