E01C0368 Human Complement Factor I ELISA kit
The Human Complement Factor I ELISA kit can be used to identify samples from the Human species. Complement Factor I can also be called AHUS3, ARMD13, C3BINA, C3b-INA, FI, IF, KAF.
Specifications of Human Complement Factor I ELISA kit
Product Information | |
Cat. No. | E01C0368 |
Product Name | Human Complement Factor I ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CFI ELISA kit uses an anti-CFI antibody and an CFI-HRP conjugate in a competitive enzyme immunoassay method. CFI-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CFI antibody binding site between CFI from samples and CFI-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CFI. Since the number of sites is limited, as more sites are occupied by CFI from the sample, fewer sites are left to bind CFI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFI concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Complement Factor I ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CFI and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Complement Factor I ELISA kit
Citations of Human Complement Factor I ELISA kit
E01C0368 has been referenced in the below publications：
The significance of IgG4 and complement factor I in idiopathic membranous nephropathy.
