Human Connective Tissue Growth Factor ELISA kit (E01C0563)

BlueGene Biotech Human Connective Tissue Growth Factor ELISA kit (E01C0563)

E01C0563 Human Connective Tissue Growth Factor ELISA kit

The Human Connective Tissue Growth Factor ELISA kit can be used to identify samples from the human species. Connective Tissue Growth Factor can also be called CCN2, HCS24, IGFBP8, NOV2, connective tissue growth factor, cellular communication network factor 2, CTGF, CCN family member 2, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8.

Products

Specifications of Human Connective Tissue Growth Factor ELISA kit

Product Information
Cat. No.	E01C0563
Product Name	Human Connective Tissue Growth Factor ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	250-5000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	250 pg/mL	1 vial
STANDARD C (0.5mL)	500 pg/mL	1 vial
STANDARD D (0.5mL)	1000 pg/mL	1 vial
STANDARD E (0.5mL)	2500 pg/mL	1 vial
STANDARD F (0.5mL)	5000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CTGF ELISA kit uses an anti-CTGF antibody and an CTGF-HRP conjugate in a competitive enzyme immunoassay method. CTGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CTGF antibody binding site between CTGF from samples and CTGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CTGF. Since the number of sites is limited, as more sites are occupied by CTGF from the sample, fewer sites are left to bind CTGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTGF concentration in each sample is interpolated from this standard curve.

Quality Control on Human Connective Tissue Growth Factor ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CTGF and analogues was observed.