E01S0079 Human Endothelial Selectin ELISA kit
The Human Endothelial Selectin ELISA kit can be used to identify samples from the human species. Endothelial Selectin can also be called E selectin, CD62E, CD62-E, E-Selectin, ELAM, ELAM1, ESEL, SEL-E, E-LAM, E-LAM1, E-SEL, LECAM2, Endothelial Leukocyte Adhesion Molecule 1, CD62 Antigen-Like Family Member E.
Product Information | |
Cat. No. | E01S0079 |
Product Name | Human Endothelial Selectin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 25 pg/mL | 1 vial |
STANDARD C (0.5mL) | 50 pg/mL | 1 vial |
STANDARD D (0.5mL) | 100 pg/mL | 1 vial |
STANDARD E (0.5mL) | 250 pg/mL | 1 vial |
STANDARD F (0.5mL) | 500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
E selectin ELISA kit uses an anti-E selectin antibody and an E selectin-HRP conjugate in a competitive enzyme immunoassay method. E selectin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-E selectin antibody binding site between E selectin from samples and E selectin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of E selectin. Since the number of sites is limited, as more sites are occupied by E selectin from the sample, fewer sites are left to bind E selectin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E selectin concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between E selectin and analogues was observed. | |
E01S0079 has been referenced in the below publications：
Research on the relationship between MMP-2, MCP-1, E-seletin, Aopps and p38MAPK signaling pathway in elderly patients with depression.
Effect of soybean isoflavones on the metabolism and anti-oxidative stress in MG-63 cells.
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