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BlueGene Biotech Human Extracellular Matrix Protein 1 ELISA kit (E01E0039)

E01E0039 Human Extracellular Matrix Protein 1 ELISA kit

The Human Extracellular Matrix Protein 1 ELISA kit can be used to identify samples from the human species. Extracellular Matrix Protein 1 can also be called ECM1, URBWD, extracellular matrix protein 1.

Products

Specifications of Human Extracellular Matrix Protein 1 ELISA kit

Product Information

Cat. No.

E01E0039

Product Name

Human Extracellular Matrix Protein 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ECM1 ELISA kit uses an anti-ECM1 antibody and an ECM1-HRP conjugate in a competitive enzyme immunoassay method. ECM1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ECM1 antibody binding site between ECM1 from samples and ECM1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ECM1. Since the number of sites is limited, as more sites are occupied by ECM1 from the sample, fewer sites are left to bind ECM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECM1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Extracellular Matrix Protein 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ECM1 and analogues was observed.


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Summary of the Assay Procedure for Human Extracellular Matrix Protein 1 ELISA kit

Summary of the Assay Procedure for Human Extracellular Matrix Protein 1 ELISA kit

Citations of Human Extracellular Matrix Protein 1 ELISA kit

E01E0039 has been referenced in the below publications:

Prognostic stratification of patients with lung, breast and liver cancers by EMT-related gene-expression profiling in tumor-adjacent tissues.

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