E01F0416 Human High affinity IgE receptor subunit alpha ELISA kit
The Human High affinity IgE receptor subunit alpha ELISA kit can be used to identify samples from the human species. High affinity IgE receptor subunit alpha can also be called FCER1A, FCE1A, FcERI, Fc-epsilon RI-alpha, IgE Fc receptor subunit alpha.
Product Information | |
Cat. No. | E01F0416 |
Product Name | Human High affinity IgE receptor subunit alpha ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FCER1A ELISA kit uses an anti-FCER1A antibody and an FCER1A-HRP conjugate in a competitive enzyme immunoassay method. FCER1A-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FCER1A antibody binding site between FCER1A from samples and FCER1A-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FCER1A. Since the number of sites is limited, as more sites are occupied by FCER1A from the sample, fewer sites are left to bind FCER1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FCER1A concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FCER1A and analogues was observed. | |
E01F0416 has been referenced in the below publications：
Mast cell activation related molecules and antibodies in differentiating allergic skin diseases.
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