E01C0087 Human High sensitivity C Reactive Protein ELISA kit
The Human High sensitivity C Reactive Protein ELISA kit can be used to identify samples from the human species. High sensitivity C Reactive Protein can also be called CRP, PTX1, C-RP, Pentraxin-Related, Pentraxin 1, C-reactive protein, pentraxin-related, C-Reactive Protein.
Product Information | |
Cat. No. | E01C0087 |
Product Name | Human High sensitivity C Reactive Protein ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Hs CRP ELISA kit uses an anti-Hs CRP antibody and an Hs CRP-HRP conjugate in a competitive enzyme immunoassay method. Hs CRP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Hs CRP antibody binding site between Hs CRP from samples and Hs CRP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Hs CRP. Since the number of sites is limited, as more sites are occupied by Hs CRP from the sample, fewer sites are left to bind Hs CRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hs CRP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Hs CRP and analogues was observed. | |
E01C0087 has been referenced in the below publications：
Association of serum immunoglobulin-G to Porphyromonas gingivalis with acute cerebral infarction in the Chinese population.
Study of Pioglitazone Reducing Coronary In-stent Restenosis on Insulin Resistance in Non-diabetic Patients.
Clinic effects of atorvastatin in non－alcoholic fatty liver disease patients and the influences on serum hsCＲP and TNF－αlevels.
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