E01I0060 Human Immunoglobulin 2Alpha ELISA kit
The Human Immunoglobulin 2Alpha ELISA kit can be used to identify samples from the human species. Immunoglobulin 2Alpha can also be called IgG2Alpha, IgG2a.
Specifications of Human Immunoglobulin 2Alpha ELISA kit
Product Information | |
Cat. No. | E01I0060 |
Product Name | Human Immunoglobulin 2Alpha ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 50 ug/mL | 1 vial |
STANDARD C (0.5mL) | 100 ug/mL | 1 vial |
STANDARD D (0.5mL) | 250 ug/mL | 1 vial |
STANDARD E (0.5mL) | 500 ug/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgG2Alpha ELISA kit uses an anti-IgG2Alpha antibody and an IgG2Alpha-HRP conjugate in a competitive enzyme immunoassay method. IgG2Alpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IgG2Alpha antibody binding site between IgG2Alpha from samples and IgG2Alpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IgG2Alpha. Since the number of sites is limited, as more sites are occupied by IgG2Alpha from the sample, fewer sites are left to bind IgG2Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG2Alpha concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Immunoglobulin 2Alpha ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgG2Alpha and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Immunoglobulin 2Alpha ELISA kit
Citations of Human Immunoglobulin 2Alpha ELISA kit
E01I0060 has been referenced in the below publications：
Differential effects of formaldehyde exposure on airway inflammation and bronchial hyperresponsiveness in BALB/c and C57BL/6 mice.
