E01I0037 Human Immunoglobulin E ELISA kit
The Human Immunoglobulin E ELISA kit can be used to identify samples from the human species. Immunoglobulin E can also be called IgE.
Specifications of Human Immunoglobulin E ELISA kit
Product Information | |
Cat. No. | E01I0037 |
Product Name | Human Immunoglobulin E ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 50 ug/mL | 1 vial |
STANDARD C (0.5mL) | 100 ug/mL | 1 vial |
STANDARD D (0.5mL) | 250 ug/mL | 1 vial |
STANDARD E (0.5mL) | 500 ug/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IgE. Standards or samples are then added to the microtiter plate wells and IgE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IgE present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IgE are added to each well to “sandwich” the IgE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IgE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgE concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Immunoglobulin E ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgE and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Immunoglobulin E ELISA kit
Citations of Human Immunoglobulin E ELISA kit
E01I0037 has been referenced in the below publications：
Mast cell activation related molecules and antibodies in differentiating allergic skin diseases.
THE ASSOCIATION ANALYSIS OF ANTI-IGE ANTIBODY AND ANTI-FCεRI ANTIBODY WITH AUTOIMMUNE LIVER DISEASE.
血清特异性过敏原IgE与过敏性皮肤病关系分析。
