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BlueGene Biotech Human Immunoglobulin E ELISA kit (E01I0037)

E01I0037 Human Immunoglobulin E ELISA kit

The Human Immunoglobulin E ELISA kit can be used to identify samples from the human species. Immunoglobulin E can also be called IgE.

Products

Specifications of Human Immunoglobulin E ELISA kit

Product Information

Cat. No.

E01I0037 

Product Name

Human Immunoglobulin E ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ug/ml

Sensitivity

1.0 ug/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

50 ug/mL

1 vial

STANDARD C (0.5mL)

100 ug/mL

1 vial

STANDARD D (0.5mL)

250 ug/mL

1 vial

STANDARD E (0.5mL)

500 ug/mL

1 vial

STANDARD F (0.5mL)

1000 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IgE. Standards or samples are then added to the microtiter plate wells and IgE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IgE present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IgE are added to each well to “sandwich” the IgE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IgE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgE concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Immunoglobulin E ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgE and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Immunoglobulin E ELISA kit

Summary of the Assay Procedure for Human Immunoglobulin E ELISA kit

Citations of Human Immunoglobulin E ELISA kit

E01I0037 has been referenced in the below publications:

Mast cell activation related molecules and antibodies in differentiating allergic skin diseases.

THE ASSOCIATION ANALYSIS OF ANTI-IGE ANTIBODY AND ANTI-FCεRI ANTIBODY WITH AUTOIMMUNE LIVER DISEASE.

血清特异性过敏原IgE与过敏性皮肤病关系分析。

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