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BlueGene Biotech Human Immunoglobulin G ELISA kit (E01I0058)

E01I0058 Human Immunoglobulin G ELISA kit

The Human Immunoglobulin G ELISA kit can be used to identify samples from the human species. Immunoglobulin G can also be called Immunoglobulin G, IgG.

Products

Specifications of Human Immunoglobulin G ELISA kit

Product Information

Cat. No.

E01I0058 

Product Name

Human Immunoglobulin G ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ug/ml

Sensitivity

1.0 ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

50 ug/mL

1 vial

STANDARD C (0.5mL)

100 ug/mL

1 vial

STANDARD D (0.5mL)

250 ug/mL

1 vial

STANDARD E (0.5mL)

500 ug/mL

1 vial

STANDARD F (0.5mL)

1000 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgG ELISA kit uses an anti-IgG antibody and an IgG-HRP conjugate in a competitive enzyme immunoassay method. IgG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IgG antibody binding site between IgG from samples and IgG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IgG. Since the number of sites is limited, as more sites are occupied by IgG from the sample, fewer sites are left to bind IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG concentration in each sample is interpolated from this standard curve.


Quality Control on Human Immunoglobulin G ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Immunoglobulin G ELISA kit

Summary of the Assay Procedure for Human Immunoglobulin G ELISA kit

Citations of Human Immunoglobulin G ELISA kit

E01I0058 has been referenced in the below publications:

The serum levels of IL-17 and IL-23 in children with Mycoplasma pneumoniae pneumonia.

The Clinical research of Ma Xing Gan Shi treating Pathogenic heat which Basing on the mode of Sequential administration.

hs-CRP与免疫功能检测在肺炎支原体肺炎诊治中的意义探讨。

Hashimoto's thyroiditis with elevated serum IgG4 concentrations is not equivalent to IgG4 Hashimoto's thyroiditis.

Exercise intervention can reduce the degree of drug dependence of patients with amphetamines/addiction by improving dopamine level and immunity and reducing negative emotions.

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