E01I0038 Human Immunoglobulin M ELISA kit
The Human Immunoglobulin M ELISA kit can be used to identify samples from the human species. Immunoglobulin M can also be called IgM, IGHM, Immunoglobulin Heavy Constant Mu, Ig mu chain C region.
Specifications of Human Immunoglobulin M ELISA kit
Product Information | |
Cat. No. | E01I0038 |
Product Name | Human Immunoglobulin M ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgM ELISA kit uses an anti-IgM antibody and an IgM-HRP conjugate in a competitive enzyme immunoassay method. IgM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IgM antibody binding site between IgM from samples and IgM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IgM. Since the number of sites is limited, as more sites are occupied by IgM from the sample, fewer sites are left to bind IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgM concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Immunoglobulin M ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgM and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Immunoglobulin M ELISA kit
Citations of Human Immunoglobulin M ELISA kit
E01I0038 has been referenced in the below publications：
Protective Effect of Bisoprolol Preconditioning on Myocardial Ischemia Reperfusion Injury in Rats by the Medium of PI3K/AKT/GS3K-b.
The serum levels of IL-17 and IL-23 in children with Mycoplasma pneumoniae pneumonia.
The Clinical research of Ma Xing Gan Shi treating Pathogenic heat which Basing on the mode of Sequential administration.
hs-CRP与免疫功能检测在肺炎支原体肺炎诊治中的意义探讨。
