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BlueGene Biotech Human Interferon alpha ELISA kit (E01I0343)

E01I0343 Human Interferon alpha ELISA kit

The Human Interferon alpha ELISA kit can be used to identify samples from the human species. Interferon alpha can also be called IFNα, IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D.

Products

Specifications of Human Interferon alpha ELISA kit

Product Information

Cat. No.

E01I0343

Product Name

Human Interferon alpha ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IFNα ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IFNα. Standards or samples are then added to the microtiter plate wells and IFNα if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFNα present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFNα are added to each well to “sandwich” the IFNα immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFNα and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNα concentration in each sample is interpolated from this standard curve.


Quality Control on Human Interferon alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IFNα and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Interferon alpha ELISA kit

Summary of the Assay Procedure for Human Interferon alpha ELISA kit

Citations of Human Interferon alpha ELISA kit

E01I0343 has been referenced in the below publications:

The study of the correlation between dendritic cell and the degree of carotid atherosclerosis and the stability of atherosclerosis plaques.

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