Human Interleukin 12 ELISA kit (E01I0033) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Interleukin 12 ELISA kit (E01I0033)

E01I0033 Human Interleukin 12 ELISA kit

The Human Interleukin 12 ELISA kit can be used to identify samples from the human species. Interleukin 12 can also be called IL12, CLMF p35, CLMF1, IL 12, CTL maturation factor (TcMF), Cytotoxic lymphocyte maturation factor 1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL 12 subunit p35, IL12A, Interleukin 12 alpha chain, Interleukin 12 p35, Interleukin 12A, Natural killer cell stimulatory factor 1, CLMF, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, IL12 subunit p35, IL12A, IL12A_HUMAN, Interleukin 12 p35, Interleukin 12A, Interleukin-12 subunit alpha, NFSK, NK cell stimulatory factor chain 1, NKSF1. NK cell stimulatory factor chain 1.

Products

Specifications of Human Interleukin 12 ELISA kit

Product Information
Cat. No.	E01I0033
Product Name	Human Interleukin 12 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IL 12 ELISA kit uses an anti- IL 12 antibody and an IL 12-HRP conjugate in a competitive enzyme immunoassay method. IL 12-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti- IL 12 antibody binding site between IL 12 from samples and IL 12-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 12. Since the number of sites is limited, as more sites are occupied by IL 12 from the sample, fewer sites are left to bind IL 12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 12 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Interleukin 12 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IL 12 and analogues was observed.