E01I0359 Human Interleukin 16 ELISA kit
The Human Interleukin 16 ELISA kit can be used to identify samples from the human species. Interleukin 16 can also be called IIL16, LCF, NPRprIL-16, IL-16, interleukin 16, IL 16, Interleukin 16 precursor, Lymphocyte Chemotactic Factor, Lymphocyte chemoattractant factor, PrIL16erleukin 16.
Product Information | |
Cat. No. | E01I0359 |
Product Name | Human Interleukin 16 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 16 ELISA kit uses an anti- IL 16 antibody and an IL 16-HRP conjugate in a competitive enzyme immunoassay method. IL 16-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti- IL 16 antibody binding site between IL 16 from samples and IL 16-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 16. Since the number of sites is limited, as more sites are occupied by IL 16 from the sample, fewer sites are left to bind IL 16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 16 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 16 and analogues was observed. | |
E01I0359 has been referenced in the below publications：
The significance of serum levels of IL-12,IL-16 and IL-17 in patients with esophageal cancer.
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