E01I0309 Human Interleukin 17 ELISA kit
The Human Interleukin 17 ELISA kit can be used to identify samples from the human species. Interleukin 17 can also be called IL17, IL 17, IL-17, Interleukin-17.
Specifications of Human Interleukin 17 ELISA kit
Product Information | |
Cat. No. | E01I0309 |
Product Name | Human Interleukin 17 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL17 ELISA kit uses an anti-IL17 antibody and a IL17-HRP conjugate in a competitive enzyme immunoassay method. IL17-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL17 antibody binding site between IL17 from samples and IL17-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL17. Since the number of sites is limited, as more sites are occupied by IL17 from the sample, fewer sites are left to bind IL17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL17 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Interleukin 17 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL17 and analogues was observed. | |
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Summary of the Assay Procedure for Human Interleukin 17 ELISA kit
Citations of Human Interleukin 17 ELISA kit
E01I0309 has been referenced in the below publications：
Clinical Evaluation of NB-UVB Combined with Tuibai Decoction for Treating Vitiligo Vulgaris in 59 Cases.
Effect of Yangyin Yifei Fang on Leukotrience B4 Level in Patients with Chronic Obstructive Pulmonary Disease in Stable Stage of Qi and Yin Deficiency Type.
Expression and clinical significance of serum IL-17 and IL-22 in patients with rheumatoid arthritis.
The Effects and Mechanisms of IL-17A in degenerated Lumbar disc inflammative reaction and cartilage degeneration in ONFH.
Study of cytokines and chest chest high-resolution CT, pulmonary function on children patients with Mycoplasma pneumoniae pneumonia.
The serum levels of IL-17 and IL-23 in children with Mycoplasma pneumoniae pneumonia.
