E01I0205 Human Interleukin 25 ELISA kit
The Human Interleukin 25 ELISA kit can be used to identify samples from the human species. Interleukin 25 can also be called IL 25, IL17E, IL17-E, Interleukin-17E, IL25, IL-25, IL-17E.
Specifications of Human Interleukin 25 ELISA kit
Product Information | |
Cat. No. | E01I0205 |
Product Name | Human Interleukin 25 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 25 ELISA kit uses an anti-IL 25 antibody and a IL 25-HRP conjugate in a competitive enzyme immunoassay method. IL 25-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 25 antibody binding site between IL 25 from samples and IL 25-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 25. Since the number of sites is limited, as more sites are occupied by IL 25 from the sample, fewer sites are left to bind IL 25-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 25 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Interleukin 25 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 25 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Interleukin 25 ELISA kit
Citations of Human Interleukin 25 ELISA kit
E01I0205 has been referenced in the below publications：
Correlation analysis of the Levels of IL-25 in blood serum with Pulmonary Function in Asthma Patients.
Curative effect of hypertonic sodium chloridehydroxyethyl starch 40 injection in the treatment of traumatic brain injury combined with hemorrhagic shock and its brain protective mechanism.
