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  • bovine serum albumin elisa 1

BlueGene Biotech Human Interleukin 6 ELISA kit (E01I0006)

E01I0006 Human Interleukin 6 ELISA kit

The Human Interleukin 6 ELISA kit can be used to identify samples from the human species. Interleukin 6 can also be called IL6, IL 6, Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, BSF2, CDF, CTL differentiation factor, Cytotoxic T cell differentiation factor, Hepatocyte stimulating factor, HGF, HPGF, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL6 protein, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin 6.

Products

Specifications of Human Interleukin 6 ELISA kit

Product Information

Cat. No.

E01I0006

Product Name

Human Interleukin 6 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 6 ELISA kit uses an anti-IL 6 antibody and a IL 6-HRP conjugate in a competitive enzyme immunoassay method. IL 6-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 6 antibody binding site between IL 6 from samples and IL 6-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 6. Since the number of sites is limited, as more sites are occupied by IL 6 from the sample, fewer sites are left to bind IL 6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 6 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Interleukin 6 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 6 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Interleukin 6 ELISA kit

Summary of the Assay Procedure for Human Interleukin 6 ELISA kit

Citations of Human Interleukin 6 ELISA kit

E01I0006 has been referenced in the below publications:

Hydrogen-rich water decreases serum low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in patients with potential metabolic syndrome.

Clinical values of urinary IL-6 in asymptomatic renal hematuria and renal hematuria with proteins.

IMM-H007, a new therapeutic candidate for nonalcoholic fatty liver disease, improves hepatic steatosis in hamsters fed a high-fat diet.

Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.

Relationships of Urinary VEGF/CR and IL-6/CR with Glomerular Pathological Injury in Asymptomatic Hematuria Patients.

Dysregulated hepatic bile acids collaboratively promote liver carcinogenesis.

Progranulin derived engineered protein Atsttrin suppresses TNF-α-mediated inflammation in intervertebral disc degenerative disease.

Mesenchymal Stem Cells Stabilize Atherosclerotic Vulnerable Plaque by Anti-Inflammatory Properties.

Anesthetic drug propofol inhibits the expression of interleukin-6, interleukin-8 and cyclooxygenase-2, a potential mechanism for propofol in suppressing tumor development and metastasis.

Casticin alleviates lipopolysaccharide-induced inflammatory responses and expression of mucus and extracellular matrix in human airway epithelial cells through Nrf2/Keap1 and NF-κB pathways.

The expression level and value of CCL-18 in the serum of the patients with Chronic Obstructive Pulmonary Disease.

Effects of Different Cryotherapy Models on Timing Sequence Recovery of Exercise Induced Muscle Damage in Middle and Long Distance Runners.

Effects of propofol on early postoperative cognitive function andinflammatory cytokines in the elderly.

脓毒症早期目标导向治疗后血乳酸清除率与血液中生物标志物的关系探讨。

Plasma 25-Hydroxyvitamin D Concentration, CRP, IL-6 and Carotid intima-media thickness Among Middle-Aged and Elderly Chinese Urban Physical Examinees.

The effects of ulinastatin on inflammatory cytokines, S100β protein and early post-operative cognitive dysfunction of patients undergoing cardiac combined valve replacement surgery.

Effect of perioperative extract of ginkgo biloba on postoperative cognitive dysfunction after non-cardiac surgery in elderly patients.

Effect of Balanced Ultrafiltration on Plasma Concentration of Antimicrobial Agents and Inflammatory Factors during Cardiac Surgery.

Effect of budesonide combined with montelukast on lung function and serum levels of IL-6,IL-8 and TNF-a in patients with chronic obstructive pulmonary disease.

In vitro study on relationship between posterior capsular opacification and materials of intraocular lenses.

Effects of IL-8 on the function of human umbilical vein endothelial cell line(HUVEC).

The effects of Berberine Hydrochloride on the vascular Endothelzal cells Injured by Glucose,Insulin and low-Denslty Lipoprotein.

Expression levels of hepcidin and interleukin-6 and their relation with anemia in multiple myeloma.

Relationship between serum S100B protein, IL-6 and white matter damage in preterm infants.

苗医药纸灸法干预肩部冷性疼痛(肩膀风)的临床研究。

Impact of depression on prognosis of patients with coronary heart disease undergoing revascularization.

Therapeutic effect of Panlong Qipian on osteoarthritis of knee joint.

重组人脑利钠肽对慢性心力衰竭患者炎症因子及血流动力学的影响。

丹红注射液对不稳定型心绞痛患者炎症因子、血栓素B2及血液流变学的影响。

The significance of changes of IL-1, IL-6, TNF-αand VEGF levels in peripheral blood of hepatocellular carcinoma with particle chemoembolization.

Circulating levels of low-density lipoprotein in psoriatic patients and its role in cytokine production.

Detection of Th17/Treg Cell-associated Cytokines in Peripheral Blood of Patients with Vitiligo and its Clinical Sig-nificance.

Clinical outcomes of continuous blood purification in patients with systemic inflammatory response syndrome.

不同剂量参附注射液治疗缓慢性心律失常的临床观察。

Influence of Astragalus Polysaccharide on the Expression of IL-6 and TNF-α of the HSP60-Induced THP -1 Macrophages.

Effect of integrated traditional Chinese and western medicine on hormone levels and inflammatory factors in patients with endometriosis.

The c‑Jun signaling pathway has a protective effect on nucleus pulposus cells in patients with intervertebral disc degeneration.

NETosis is associated with the severity of aortic stenosis: Links with inflammation.

Prunetin inhibits lipopolysaccharide-induced inflammatory cytokine production and MUC5AC expression by inactivating the TLR4/MyD88 pathway in human nasal epithelial cells.

Tripterine alleviates lipopolysaccharide-induced airway epithelial barrier dysfunction through suppressing the Hippo pathway.

Comparison of Pancreatic Microcirculation Profiles in Spontaneously Hypertensive Rats and Wistar-Kyoto Rats by Laser Doppler and Wavelet Transform Analysis.

Polyinosinic-polycytidylic acid induces innate immune responses via Toll like receptor 3 in human ovarian granulosa cells.

Adiponectin antagonises LPS-regulated secretion of inflammatory factors in airway epithelial cells, and its expression is regulated by many factors.

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