E01I0056 Human Interleukin 8 ELISA kit
The Human Interleukin 8 ELISA kit can be used to identify samples from the human species. Interleukin 8 can also be called CXCL8, IL 8, chemokine (C-X-C motif) ligand 8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, IL8, C-X-C motif chemokine ligand 8, Interleukin-8.
Product Information | |
Cat. No. | E01I0056 |
Product Name | Human Interleukin 8 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 8 ELISA kit uses an anti-IL 8 antibody and a IL 8-HRP conjugate in a competitive enzyme immunoassay method. IL 8-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 8 antibody binding site between IL 8 from samples and IL 8-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 8. Since the number of sites is limited, as more sites are occupied by IL 8 from the sample, fewer sites are left to bind IL 8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 8 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 8 and analogues was observed. | |
E01I0056 has been referenced in the below publications：
An important role of interleukin-10 in counteracting excessive immune response in HT-29 cells exposed to Clostridium butyricum.
Clostridium butyricum activates TLR2-mediated MyD88-independent signaling pathway in HT-29 cells.
Anesthetic drug propofol inhibits the expression of interleukin-6, interleukin-8 and cyclooxygenase-2, a potential mechanism for propofol in suppressing tumor development and metastasis.
Casticin alleviates lipopolysaccharide-induced inflammatory responses and expression of mucus and extracellular matrix in human airway epithelial cells through Nrf2/Keap1 and NF-κB pathways.
The Clinical Study on the effect of Kun Fu Kang Capsule on Red Cell Immunology with Chronic Pelvic Inflammatory Disease.
Study on Molecular Mechanism of the Interaction between Clostridium butyricum and Intestinal epithelial cells.
Effects of atorvastatin and fluvastatin on blood levels of blood-serum inflammatory cytokines and lipids in patients with unstable angina undergoing percutaneous coronary intervention.
脓毒症早期目标导向治疗后血乳酸清除率与血液中生物标志物的关系探讨。
Effect of tirofiban on IL-8/IL-10 level after PCl in coronary heart disease patients.
Clinical Study of 101 Patients with Primary Bronchogenic Carcinoma.
Study on the proliferation of leukemia cell line(HL-60) stimulated by IL-8 from bone marrow stromal cells of the children with acute myeloid leukemia.
Effect of tirofiban on IL-8/IL-10 level after PCI in coronary heart disease patients.
Clinical outcomes of continuous blood purification in patients with systemic inflammatory response syndrome.
Prunetin inhibits lipopolysaccharide-induced inflammatory cytokine production and MUC5AC expression by inactivating the TLR4/MyD88 pathway in human nasal epithelial cells.
Tripterine alleviates lipopolysaccharide-induced airway epithelial barrier dysfunction through suppressing the Hippo pathway.
Adiponectin antagonises LPS-regulated secretion of inflammatory factors in airway epithelial cells, and its expression is regulated by many factors.
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