Human Kidney Injury Molecule 1 ELISA kit (E01K0025) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Kidney Injury Molecule 1 ELISA kit (E01K0025)

E01K0025 Human Kidney Injury Molecule 1 ELISA kit

The Human Kidney Injury Molecule 1 ELISA kit can be used to identify samples from the human species. Kidney Injury Molecule 1 can also be called HAVCR1, HAVcr-1, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1, CD365, KIM-1 Kidney Injury Molecule -1, T-cell immunoglobulin and mucin domain 1, KIM-1 Kidney Injury Molecule -1, T-cell immunoglobulin and mucin domain 1, Hepatitis A Virus Cellular Receptor 1, T Cell Immunoglobulin And Mucin Domain-Containing Protein 1, T-cell immunoglobulin mucin receptor 1.

Products

Specifications of Human Kidney Injury Molecule 1 ELISA kit

Product Information
Cat. No.	E01K0025
Product Name	Human Kidney Injury Molecule 1 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

KIM1 ELISA kit uses an anti-KIM1 antibody and an KIM1-HRP conjugate in a competitive enzyme immunoassay method. KIM1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KIM1 antibody binding site between KIM1 from samples and KIM1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KIM1. Since the number of sites is limited, as more sites are occupied by KIM1 from the sample, fewer sites are left to bind KIM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KIM1 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Kidney Injury Molecule 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between KIM1 and analogues was observed.