E01L0289 Human Leukotriene E4 ELISA kit
The Human Leukotriene E4 ELISA kit can be used to identify samples from the human species. Leukotriene E4 can also be called LT E4, LT-E4.
Specifications of Human Leukotriene E4 ELISA kit
Product Information | |
Cat. No. | E01L0289 |
Product Name | Human Leukotriene E4 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LT E4 ELISA kit uses an anti-LT E4 antibody and an LT E4-HRP conjugate in a competitive enzyme immunoassay method. LT E4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LT E4 antibody binding site between LT E4 from samples and LT E4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LT E4. Since the number of sites is limited, as more sites are occupied by LT E4 from the sample, fewer sites are left to bind LT E4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LT E4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Leukotriene E4 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LT E4 and analogues was observed. | |
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Summary of the Assay Procedure for Human Leukotriene E4 ELISA kit
Citations of Human Leukotriene E4 ELISA kit
E01L0289 has been referenced in the below publications：
Detection and Analysis of Leukotriene E4 and Tumor Necrosis Factor of Patients with Seasonal Allergic Rhinitis.
