Human Macrophage Inflammatory Protein 1β ELISA kit (E01M0206)

BlueGene Biotech Human Macrophage Inflammatory Protein 1β ELISA kit (E01M0206)

E01M0206 Human Macrophage Inflammatory Protein 1β ELISA kit

The Human Macrophage Inflammatory Protein 1β ELISA kit can be used to identify samples from the human species. Macrophage Inflammatory Protein 1β can also be called MIP1β, CCL4L1, MIP-1β, AT744.2, CCL4L, LAG-1, LAG1, SCYA4L, SCYA4L1, MIP-1-beta, SCYA4L2, C-C motif chemokine ligand 4 like 1, Chemokine (C-C motif) ligands 4, CCL4, ACT2, G-26, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b.

Products

Specifications of Human Macrophage Inflammatory Protein 1β ELISA kit

Product Information
Cat. No.	E01M0206
Product Name	Human Macrophage Inflammatory Protein 1β ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MIP1β ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MIP1β. Standards or samples are then added to the microtiter plate wells and MIP1β if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MIP1β present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MIP1β are added to each well to “sandwich” the MIP1β immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MIP1β and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP1β concentration in each sample is interpolated from this standard curve.

Quality Control on Human Macrophage Inflammatory Protein 1β ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MIP1β and analogues was observed.