Human Macrophage Inflammatory Protein 1a ELISA kit (E01M0004)

BlueGene Biotech Human Macrophage Inflammatory Protein 1a ELISA kit (E01M0004)

E01M0004 Human Macrophage Inflammatory Protein 1a ELISA kit

The Human Macrophage Inflammatory Protein 1a ELISA kit can be used to identify samples from the human species. Macrophage Inflammatory Protein 1a can also be called CCL3, MIP1-A, MIP-1α, MIP1a, SCYA3, Chemokine C-C-Motif Ligand 3, Small Inducible Cytokine A3, Homologous To Mouse Mip-1a, Tonsillar Lymphocyte LD78 Alpha Protein.

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Specifications of Human Macrophage Inflammatory Protein 1a ELISA kit

Product Information
Cat. No.	E01M0004
Product Name	Human Macrophage Inflammatory Protein 1a ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MIP1a ELISA kit uses an anti-MIP1a antibody and a MIP1a-HRP conjugate in a competitive enzyme immunoassay method. MIP1a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MIP1a antibody binding site between MIP1a from samples and MIP1a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MIP1a. Since the number of sites is limited, as more sites are occupied by MIP1a from the sample, fewer sites are left to bind MIP1a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP1a concentration in each sample is interpolated from this standard curve.

Quality Control on Human Macrophage Inflammatory Protein 1a ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MIP1a and analogues was observed.

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Summary of the Assay Procedure for Human Macrophage Inflammatory Protein 1a ELISA kit

Citations of Human Macrophage Inflammatory Protein 1a ELISA kit

E01M0004 has been referenced in the below publications：

Expression and clinical significance of macrophage inflammatory protein-1a,interferon gamma inducible protein 10 and angiopoietin-1in primary acute myelogenous leukemia.