E01M0368 Human Mast Cell Chymase ELISA kit
The Human Mast Cell Chymase ELISA kit can be used to identify samples from the human species. Mast Cell Chymase can also be called MCC.
Specifications of Human Mast Cell Chymase ELISA kit
Product Information | |
Cat. No. | E01M0368 |
Product Name | Human Mast Cell Chymase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MCC ELISA kit uses an anti-MCC antibody and an MCC-HRP conjugate in a competitive enzyme immunoassay method. MCC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MCC antibody binding site between MCC from samples and MCC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MCC. Since the number of sites is limited, as more sites are occupied by MCC from the sample, fewer sites are left to bind MCC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCC concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Mast Cell Chymase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MCC and analogues was observed. | |
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Summary of the Assay Procedure for Human Mast Cell Chymase ELISA kit
Citations of Human Mast Cell Chymase ELISA kit
E01M0368 has been referenced in the below publications：
Dengue virus-elicited tryptase induces endothelial permeability and shock.
Serum chymase levels correlate with severe dengue warning signs and clinical fuid accumulation in hospitalized pediatric patients.
Chymase Level Is a Predictive Biomarker of Dengue Hemorrhagic Fever in Pediatric and Adult Patients.
