E01C0613 Human Neopterin ELISA kit
The Human Neopterin ELISA kit can be used to identify samples from the human species. Neopterin can also be called 2-Amino-6-(1,2,3-trihydroxypropyl)pteridin-4(1H)-one.
Specifications of Human Neopterin ELISA kit
Product Information | |
Cat. No. | E01N0603 |
Product Name | Human Neopterin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 nmol/L |
Sensitivity | 1.0 nmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/L | 1 vial |
STANDARD B (0.5mL) | 5.0 nmol/L | 1 vial |
STANDARD C (0.5mL) | 10 nmol/L | 1 vial |
STANDARD D (0.5mL) | 25 nmol/L | 1 vial |
STANDARD E (0.5mL) | 50 nmol/L | 1 vial |
STANDARD F (0.5mL) | 100 nmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NP ELISA kit uses an anti-NP antibody and an NP-HRP conjugate in a competitive enzyme immunoassay method. NP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NP antibody binding site between NP from samples and NP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NP. Since the number of sites is limited, as more sites are occupied by NP from the sample, fewer sites are left to bind NP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NP concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Cyclophilin A ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Neopterin ELISA kit
Citations of Human Neopterin ELISA kit
E01N0603 has been referenced in the below publications：
Inflammatory Bowel Disease-Associated Changes in the Gut:Focus on Kazan Patients.
