Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit (E01P0023)

BlueGene Biotech Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit (E01P0023)

E01P0023 Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

The Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit can be used to identify samples from the human species. Phosphorylation P38 Mitogen activated Protein Kinase can also be called P P38MAPK.

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Specifications of Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Product Information
Cat. No.	E01P0023
Product Name	Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	25-500 ng/ml
Sensitivity	1.0 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	25 ng/mL	1 vial
STANDARD C (0.5mL)	50 ng/mL	1 vial
STANDARD D (0.5mL)	100 ng/mL	1 vial
STANDARD E (0.5mL)	250 ng/mL	1 vial
STANDARD F (0.5mL)	500 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

P P38MAPK ELISA kit uses an anti-P P38MAPK antibody and an P P38MAPK-HRP conjugate in a competitive enzyme immunoassay method. P P38MAPK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-P P38MAPK antibody binding site between P P38MAPK from samples and P P38MAPK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of P P38MAPK. Since the number of sites is limited, as more sites are occupied by P P38MAPK from the sample, fewer sites are left to bind P P38MAPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P P38MAPK concentration in each sample is interpolated from this standard curve.

Quality Control on Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between P P38MAPK and analogues was observed.

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Summary of the Assay Procedure for Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Citations of Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

E01P0023 has been referenced in the below publications：

Dissertation Submitted to Guangzhou Sport University for Master Degree.