Human Platelet Activating Factor Acetylhydrolase ELISA kit (E01P0573)

BlueGene Biotech Human Platelet Activating Factor Acetylhydrolase ELISA kit (E01P0573)

E01P0573 Human Platelet Activating Factor Acetylhydrolase ELISA kit

The Human Platelet Activating Factor Acetylhydrolase ELISA kit can be used to identify samples from the human species. Platelet Activating Factor Acetylhydrolase can also be called SD-PLA, Serine-dependent phospholipase A, Platelet-activating factor acetylhydrolase, cytoplasmic.

Products

Specifications of Human Platelet Activating Factor Acetylhydrolase ELISA kit

Product Information
Cat. No.	E01P0573
Product Name	Human Platelet Activating Factor Acetylhydrolase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	2.5-50ng/mL
Sensitivity	0.1 ng/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

PAFAH ELISA kit uses an anti-PAFAH antibody and an PAFAH-HRP conjugate in a competitive enzyme immunoassay method. PAFAH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PAFAH antibody binding site between PAFAH from samples and PAFAH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PAFAH. Since the number of sites is limited, as more sites are occupied by PAFAH from the sample, fewer sites are left to bind PAFAH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAFAH concentration in each sample is interpolated from this standard curve.

Quality Control on Human Platelet Activating Factor Acetylhydrolase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between PAFAH and analogues was observed.