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BlueGene Biotech Human Platelet activating factor ELISA kit (E01P0589)

E01P0589 Human Platelet activating factor ELISA kit

The Human Platelet activating factor ELISA kit can be used to identify samples from the human species. Platelet activating factor can also be called PAF, PAF-Acether, AGEPC, Acetyl-Glyceryl-Ether-Phosphorylcholine, acetyl-glyceryl-ether-phosphorylcholine.

Products

Specifications of Human Platelet activating factor ELISA kit

Product Information

Cat. No.

E01P0589 

Product Name

Human Platelet activating factor ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PAF ELISA kit uses an anti-PAF antibody and an PAF-HRP conjugate in a competitive enzyme immunoassay method. PAF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PAF antibody binding site between PAF from samples and PAF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PAF. Since the number of sites is limited, as more sites are occupied by PAF from the sample, fewer sites are left to bind PAF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAF concentration in each sample is interpolated from this standard curve.


Quality Control on Human Platelet activating factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PAF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Platelet activating factor ELISA kit

Summary of the Assay Procedure for Human Platelet activating factor ELISA kit

Citations of Human Platelet activating factor ELISA kit

E01P0589 has been referenced in the below publications:

Effects of Local Mild Hypothermia Therapy On Serum Content of Platelet Activating Factor in Patients with Acute Large Area Cerebral Infarction.

Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation.

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