E01P0037 Human Platelet activating factor receptor ELISA kit
The Human Platelet activating factor receptor ELISA kit can be used to identify samples from the human species. Platelet activating factor receptor can also be called PTAFR.
Specifications of Human Platelet activating factor receptor ELISA kit
Product Information | |
Cat. No. | E01P0037 |
Product Name | Human Platelet activating factor receptor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PAFR ELISA kit uses an anti-PAFR antibody and an PAFR-HRP conjugate in a competitive enzyme immunoassay method. PAFR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PAFR antibody binding site between PAFR from samples and PAFR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PAFR. Since the number of sites is limited, as more sites are occupied by PAFR from the sample, fewer sites are left to bind PAFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAFR concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Platelet activating factor receptor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PAFR and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Platelet activating factor receptor ELISA kit
Citations of Human Platelet activating factor receptor ELISA kit
E01P0037 has been referenced in the below publications：
SIRT1 prevents pulmonary thrombus formation induced by arachidonic acid via downregulation of PAF receptor expression in platelets.
