E01P0072 Human Platelet Derived Growth Factor Subunit A ELISA kit
The Human Platelet Derived Growth Factor Subunit A ELISA kit can be used to identify samples from the human species. Platelet Derived Growth Factor Subunit A can also be called PDGFA, PDGF-A, PDGF1, Platelet Derived Growth Factor Alpha Polypeptide.
Product Information | |
Cat. No. | E01P0072 |
Product Name | Human Platelet Derived Growth Factor Subunit A ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 25 pg/mL | 1 vial |
STANDARD C (0.5mL) | 50 pg/mL | 1 vial |
STANDARD D (0.5mL) | 100 pg/mL | 1 vial |
STANDARD E (0.5mL) | 250 pg/mL | 1 vial |
STANDARD F (0.5mL) | 500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PDGFA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDGFA. Standards or samples are then added to the microtiter plate wells and PDGFA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PDGFA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PDGFA are added to each well to “sandwich” the PDGFA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PDGFA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGFA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PDGFA and analogues was observed. | |
E01P0072 has been referenced in the below publications：
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer’s Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus.
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