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BlueGene Biotech Human sCD14 ELISA kit (E01C0876)

E01C0876 Human sCD14 ELISA Kit

The Human sCD14 ELISA kit can be used to identify samples from the human species. sCD14 can also be called sentrez:929, SCD14 molecule.

Products

Specifications of Human sCD14 ELISA kit

Product Information

Cat. No.

E01C0876

Product Name

Human sCD14 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

sCD14 ELISA kit uses an anti-sCD14 antibody and an sCD14-HRP conjugate in a competitive enzyme immunoassay method. sCD14-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sCD14 antibody binding site between sCD14 from samples and sCD14-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sCD14. Since the number of sites is limited, as more sites are occupied by sCD14 from the sample, fewer sites are left to bind sCD14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD14 concentration in each sample is interpolated from this standard curve.


Quality Control on Human sCD14 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between sCD14 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human sCD14 ELISA kit

Summary of the Assay Procedure for Human sCD14 ELISA kit

Citations of Human sCD14 ELISA kit

E01C0876 has been referenced in the below publications:

Markers of the innate immune system in the cerebrospinal fluid in patients with severe depression.

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