E01C0053 Human Soluble CD 44v9 ELISA kit
The Human Soluble CD 44v9 ELISA kit can be used to identify samples from the human species. Soluble CD 44v9 can also be called Soluble CD44 variant 9, Soluble CD44 isoform 9.
Specifications of Human Soluble CD 44v9 ELISA kit
Product Information | |
Cat. No. | E01C0053 |
Product Name | Human Soluble CD 44v9 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SCD44V9 ELISA kit uses an anti-SCD44V9 antibody and an SCD44V9-HRP conjugate in a competitive enzyme immunoassay method. SCD44V9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SCD44V9 antibody binding site between SCD44V9 from samples and SCD44V9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SCD44V9. Since the number of sites is limited, as more sites are occupied by SCD44V9 from the sample, fewer sites are left to bind SCD44V9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCD44V9 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Soluble CD 44v9 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SCD44V9 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Soluble CD 44v9 ELISA kit
Citations of Human Soluble CD 44v9 ELISA kit
E01C0053 has been referenced in the below publications：
Silencing Effect of siRNA on FAK Geng of Human Laryngeal Carcinoma Hep-2 Cell Line.
