E01S0230 Human Soluble CD36 ELISA kit
The Human Soluble CD36 ELISA kit can be used to identify samples from the human species. Soluble CD36 can also be called sBDPLT10, sCHDS7, sFAT, sGP3B, sGP4,sGPIV, sPASIV, sSCARB3, sCD36 molecule.
Specifications of Human Soluble CD36 ELISA kit
Product Information | |
Cat. No. | E01S0230 |
Product Name | Human Soluble CD36 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sCD36 ELISA kit uses an anti-sCD36 antibody and an sCD36-HRP conjugate in a competitive enzyme immunoassay method. sCD36-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sCD36 antibody binding site between sCD36 from samples and sCD36-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sCD36. Since the number of sites is limited, as more sites are occupied by sCD36 from the sample, fewer sites are left to bind sCD36-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD36 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Soluble CD36 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sCD36 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Soluble CD36 ELISA kit
Citations of Human Soluble CD36 ELISA kit
E01S0230 has been referenced in the below publications：
Plasma soluble CD36, carotid intima-media thickness and cognitive function in patients with type 2 diabetes.
Frequency of CD36 deficiency in Thais analyzed by quantification of CD36 on cell surfaces and in plasma.
