E01S0291 Human Soluble transferrin receptor ELISA kit
The Human Soluble transferrin receptor ELISA kit can be used to identify samples from the human species. Soluble transferrin receptor can also be called sCD71,sTFR1,sP90,sTFRC,sT9,sTrfr,sTfR,sTransferrin receptor protein 1, sserum form.
Product Information | |
Cat. No. | E01S0291 |
Product Name | Human Soluble transferrin receptor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sTfR ELISA kit uses an anti-sTfR antibody and an sTfR-HRP conjugate in a competitive enzyme immunoassay method. sTfR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sTfR antibody binding site between sTfR from samples and sTfR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sTfR. Since the number of sites is limited, as more sites are occupied by sTfR from the sample, fewer sites are left to bind sTfR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sTfR concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sTfR and analogues was observed. | |
E01S0291 has been referenced in the below publications：
Dissertation Submitted to Guangzhou Sport University for Master Degree.
Acupuncture Improves Intestinal Absorption of Iron in Iron‑deficient Obese Patients: A Randomized Controlled Preliminary Trial.
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