E01S0295 Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA
The Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA can be used to identify samples from the human species. Soluble tumor necrosis factor-related apoptosis inducing ligand can also be called sTNFSF10, sAPO2L, sApo-2L, sCD253, TL2, sTRAIL, sTNLG6A, stumor necrosis factor superfamily member 10, sTNF superfamily member 10.
Product Information | |
Cat. No. | E01S0295 |
Product Name | Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Strail ELISA kit uses an anti-Strail antibody and an Strail-HRP conjugate in a competitive enzyme immunoassay method. Strail-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Strail antibody binding site between Strail from samples and Strail-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Strail. Since the number of sites is limited, as more sites are occupied by Strail from the sample, fewer sites are left to bind Strail-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Strail concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Strail and analogues was observed. | |
E01S0295 has been referenced in the below publications：
Effects of Entecavir on serum sTRAIL in patients with hepatitis B virus-related cirrhosis.
Related Bluegene Biotech Products
BlueGene Biotech News
