Name: Human Surfactant Protein J ELISA kit (E01S0096)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Human Surfactant Protein J ELISA kit (E01S0096)

E01S0096 Human Surfactant Protein J ELISA kit

The Human Surfactant Protein J ELISA kit can be used to identify samples from the human species. Surfactant Protein J can also be called SPJ, SPG, Surfactant Associated Protein G, Surfactant-associated protein 2, SFTA2.

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Specifications of Human Surfactant Protein J ELISA kit

Product Information
Cat. No.	E01S0096
Product Name	Human Surfactant Protein J ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/mL
Sensitivity	0.1 ng/mL
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SPJ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SPJ. Standards or samples are then added to the microtiter plate wells and SPJ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SPJ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SPJ are added to each well to “sandwich” the SPJ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SPJ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPJ concentration in each sample is interpolated from this standard curve.

Quality Control on Human Surfactant Protein J ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	91-105%
Linearity	Diluent Ratio	Range %
	1:2	88-106
	1:4	87-108
	1:8	85-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SPJ and analogues was observed.

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Summary of the Assay Procedure for Human Surfactant Protein J ELISA kit

Citations of Human Surfactant Protein J ELISA kit

E01S0096 has been referenced in the below publications:

Surfactant proteins A and D are related to severity of the disease, pathogenic bacteria and comorbidity in patients with chronic rhinosinusitis with and without nasal polyps.