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BlueGene Biotech Human Total Immunoglobulin ELISA kit (E01T0019)

E01T0019 Human Total Immunoglobulin ELISA kit

The Human Total Immunoglobulin ELISA kit can be used to identify samples from the human species. Total Immunoglobulin can also be called Immunoglobulin.

Products

Specifications of Human Total Immunoglobulin ELISA kit

Product Information

Cat. No.

E01T0019

Product Name

Human Total Immunoglobulin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 mg/ml

Sensitivity

1.0 mg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/mL

1 vial

STANDARD B (0.5mL)

5.0 mg/mL

1 vial

STANDARD C (0.5mL)

10 mg/mL

1 vial

STANDARD D (0.5mL)

25 mg/mL

1 vial

STANDARD E (0.5mL)

50 mg/mL

1 vial

STANDARD F (0.5mL)

100 mg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TIG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TIG antibody and an TIG-HRP conjugate. The assay sample and buffer are incubated together with TIG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TIG concentration since TIG from samples and TIG-HRP conjugate compete for the anti-TIG antibody binding site. Since the number of sites is limited, as more sites are occupied by TIG from the sample, fewer sites are left to bind TIG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIG concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Total Immunoglobulin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TIG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Total Immunoglobulin ELISA kit

Summary of the Assay Procedure for Human Total Immunoglobulin ELISA kit

Citations of Human Total Immunoglobulin ELISA kit

E01T0019 has been referenced in the below publications:

A role for gut-associated lymphoid tissue in shaping the human B cell repertoire.

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