E01T0034 Human Total tumor necrosis factor ELISA kit
The Human Total tumor necrosis factor ELISA kit can be used to identify samples from the human species. Total tumor necrosis factor can also be called TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, TNF-a, tumor necrosis factor, TNLG1F, Tumor necrosis factor alpha.
Product Information | |
Cat. No. | E01T0034 |
Product Name | Human Total tumor necrosis factor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TNF ELISA kit uses an anti-TNF antibody and an TNF-HRP conjugate in a competitive enzyme immunoassay method. TNF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNF antibody binding site between TNF from samples and TNF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNF. Since the number of sites is limited, as more sites are occupied by TNF from the sample, fewer sites are left to bind TNF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TNF and analogues was observed. | |
E01T0034 has been referenced in the below publications：
Therapeutic effect of Panlong Qipian on osteoarthritis of knee joint.
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