Human Tumor Necrosis Factor β ELISA kit (E01T0021) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Tumor Necrosis Factor β ELISA kit (E01T0021)

E01T0021 Human Tumor Necrosis Factor β ELISA kit

The Human Transglutaminase ELISA kit can be used to identify samples from the human species. Transglutaminase can also be called TNFβ, LTA, LT, TNFB, TNFSF1, Lymphotoxin alpha, TNLG1E, TNF-B, Tumor Necrosis Factor Ligand Superfamily Member 1,Lymphotoxin Alpha.

Products

Specifications of Human Tumor Necrosis Factor β ELISA kit

Product Information
Cat. No.	E01T0021
Product Name	Human Tumor Necrosis Factor β ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

TNFβ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TNFβ. Standards or samples are then added to the microtiter plate wells and TNFβ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TNFβ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TNFβ are added to each well to “sandwich” the TNFβ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNFβ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFβ concentration in each sample is interpolated from this standard curve.

Quality Control on Human Tumor Necrosis Factor β ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between TNFβ and analogues was observed.