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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Mannma binding protein/mannan binding lectin ELISA kit (E14M0240)

E14M0240 Sheep Mannma binding protein/mannan binding lectin ELISA kit

Sheep Mannma binding protein/mannan binding lectin ELISA kit is suitable for the detection of samples from sheep species. Mannma binding protein/mannan binding lectin can also be called MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, MBL.

Products

Specifications of Sheep Mannma binding protein/mannan binding lectin ELISA kit

Product Information

Cat. No.

E14M0240

Product Name

Sheep Mannma binding protein/mannan binding lectin ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MBP/MBL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MBP/MBL antibody and an MBP/MBL-HRP conjugate. The assay sample and buffer are incubated together with MBP/MBL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBP/MBL concentration since MBP/MBL from samples and MBP/MBL-HRP conjugate compete for the anti-MBP/MBL antibody binding site. Since the number of sites is limited, as more sites are occupied by MBP/MBL from the sample, fewer sites are left to bind MBP/MBL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP/MBL concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Mannma binding protein/mannan binding lectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MBP/MBL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Mannma binding protein/mannan binding lectin ELISA kit

Summary of the Assay Procedure for Sheep Mannma binding protein/mannan binding lectin ELISA kit

Citations of Sheep Mannma binding protein/mannan binding lectin ELISA kit

E14M0240 has been referenced in the below publications:

Construction of Sheep MBL Eukaryotic Expression Vector and Screening of disease resistance associated miRNA.

Study on the relationship between sheep Mannose—binding lectin gene promoter methylation and sheep Mycoplasma pneumoniae infection.

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