Name: Sheep Mannma binding protein/mannan binding lectin ELISA kit (E14M0240)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Sheep Mannma binding protein/mannan binding lectin ELISA kit (E14M0240)

E14M0240 Sheep Mannma binding protein/mannan binding lectin ELISA kit

Sheep Mannma binding protein/mannan binding lectin ELISA kit is suitable for the detection of samples from sheep species. Mannma binding protein/mannan binding lectin can also be called MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, MBL.

Products

Specifications of Sheep Mannma binding protein/mannan binding lectin ELISA kit

Product Information
Cat. No.	E14M0240
Product Name	Sheep Mannma binding protein/mannan binding lectin ELISA kit
Species	Sheep
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MBP/MBL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MBP/MBL antibody and an MBP/MBL-HRP conjugate. The assay sample and buffer are incubated together with MBP/MBL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBP/MBL concentration since MBP/MBL from samples and MBP/MBL-HRP conjugate compete for the anti-MBP/MBL antibody binding site. Since the number of sites is limited, as more sites are occupied by MBP/MBL from the sample, fewer sites are left to bind MBP/MBL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP/MBL concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Mannma binding protein/mannan binding lectin ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	87-105%
Linearity	Diluent Ratio	Range %
	1:2	84-108
	1:4	81-107
	1:8	80-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MBP/MBL and analogues was observed.

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Summary of the Assay Procedure for Sheep Mannma binding protein/mannan binding lectin ELISA kit

Citations of Sheep Mannma binding protein/mannan binding lectin ELISA kit

E14M0240 has been referenced in the below publications:

Construction of Sheep MBL Eukaryotic Expression Vector and Screening of disease resistance associated miRNA.

Study on the relationship between sheep Mannose—binding lectin gene promoter methylation and sheep Mycoplasma pneumoniae infection.