Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit (E03M0246)

BlueGene Biotech Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit (E03M0246)

E03M0246 Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit

The Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit can be used to identify samples from the mouse species. Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor can also be called C-C motif chemokine 2, CCL2, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je), Small inducible cytokine A2, Small inducible cytokine subfamily A (Cys Cys), member 2, Small inducible cytokine subfamily A Cys Cys member 2, Small-inducible cytokine A2, SMCCF.

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Specifications of Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit

Product Information
Cat. No.	E03M0246
Product Name	Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MCP 1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MCP 1 antibody and an MCP 1-HRP conjugate. The assay sample and buffer are incubated together with MCP 1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MCP 1 concentration since MCP 1 from samples and MCP 1-HRP conjugate compete for the anti-MCP 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MCP 1 from the sample, fewer sites are left to bind MCP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MCP 1 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MCP 1 and analogues was observed.

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Summary of the Assay Procedure for Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit

Citations of Mouse Monocyte Chemotactic Protein 1/Monocyte Chemotactic and Activating Factor ELISA kit

E03M0246 has been referenced in the below publications：

高同型半胱氨酸血症经NFK/DNMT1诱导APoE鼠MCP-1DNA低甲基化致动脉粥样硬化的研究。