E03S0263 Mouse Anti Soluble Liver Antigen ELISA kit
Mouse Anti Soluble Liver Antigen ELISA kit is suitable for detecting samples from mouse species. Anti Soluble Liver Antigen can also be called SEPSECS, LP, PCH2D, SLA, SLA/LP, Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase.
Product Information | |
Cat. No. | E03S0263 |
Product Name | Mouse Anti Soluble Liver Antigen ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100ng/ml |
Sensitivity | 1.0ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SLAAb ELISA kit applies the competitive enzyme immunoassay technique utilizing an soluble Liver antigen and an SLAAb-HRP conjugate. The assay sample and buffer are incubated together with SLAAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLAAb concentration since SLAAb from samples and SLAAb-HRP conjugate compete for the soluble Liver antigen binding site. Since the number of sites is limited, as more sites are occupied by SLAAb from the sample, fewer sites are left to bind SLAAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLAAb concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 98-104% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-103 | |
1:4 | 95-107 | |
1:8 | 91-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SLA and analogues was observed. | |
E03S0263 has been referenced in the below publications:
Medullary thymic epithelial cell depletion leads to autoimmune hepatitis.
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