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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Anti Soluble Liver Antigen ELISA kit (E03S0263)

E03S0263 Mouse Anti Soluble Liver Antigen ELISA kit

Mouse Anti Soluble Liver Antigen ELISA kit is suitable for detecting samples from mouse species. Anti Soluble Liver Antigen can also be called  SEPSECS, LP, PCH2D, SLA, SLA/LP, Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase.

Products

Specifications of Mouse Anti Soluble Liver Antigen ELISA kit

Product Information

Cat. No.

E03S0263

Product Name

Mouse Anti Soluble Liver Antigen ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5-100ng/ml

Sensitivity

1.0ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SLAAb ELISA kit applies the competitive enzyme immunoassay technique utilizing an soluble Liver antigen and an SLAAb-HRP conjugate. The assay sample and buffer are incubated together with SLAAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLAAb concentration since SLAAb from samples and SLAAb-HRP conjugate compete for the soluble Liver antigen binding site. Since the number of sites is limited, as more sites are occupied by SLAAb from the sample, fewer sites are left to bind SLAAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLAAb concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Anti Soluble Liver Antigen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

98-104%

Linearity

Diluent Ratio

Range %

1:2

96-103

1:4

95-107

1:8

91-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SLA and analogues was observed.


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Summary of the Assay Procedure for Mouse Anti Soluble Liver Antigen ELISA kit

Summary of the Assay Procedure for Mouse Anti Soluble Liver Antigen ELISA kit

Citations of Mouse Anti Soluble Liver Antigen ELISA kit

E03S0263 has been referenced in the below publications:

Medullary thymic epithelial cell depletion leads to autoimmune hepatitis.

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