Mouse Cutaneous T Cell Attracting Chemokine ELISA kit (E03C0595)

BlueGene Biotech Mouse Cutaneous T Cell Attracting Chemokine ELISA kit (E03C0595)

E03C0595 Mouse Cutaneous T Cell Attracting Chemokine ELISA kit

The Mouse Cutaneous T Cell Attracting Chemokine ELISA kit can be used to identify samples from the mouse species. Cutaneous T Cell Attracting Chemokine can also be called ALP, ILC, CTAK, CTACK, PESKY, ESKINE, SCYA27．

Products

Specifications of Mouse Cutaneous T Cell Attracting Chemokine ELISA kit

Product Information
Cat. No.	E03C0595
Product Name	Mouse Cutaneous T Cell Attracting Chemokine ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CTACK ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CTACK antibody and an CTACK-HRP conjugate. The assay sample and buffer are incubated together with CTACK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTACK concentration since CTACK from samples and CTACK-HRP conjugate compete for the anti-CTACK antibody binding site. Since the number of sites is limited, as more sites are occupied by CTACK from the sample, fewer sites are left to bind CTACK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTACK concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Cutaneous T Cell Attracting Chemokine ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CTACK and analogues was observed.